DDX42 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87135

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX42 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX42 Antibody - BSA Free

Western Blot: DDX42 Antibody [NBP1-87135]

Western Blot: DDX42 Antibody [NBP1-87135]

Western Blot: DDX42 Antibody [NBP1-87135] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: DDX42 Antibody [NBP1-87135]

Immunocytochemistry/ Immunofluorescence: DDX42 Antibody [NBP1-87135]

Immunocytochemistry/Immunofluorescence: DDX42 Antibody [NBP1-87135] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135] - Staining of human tonsil shows moderate nuclear positivity.
Western Blot: DDX42 Antibody [NBP1-87135]

Western Blot: DDX42 Antibody [NBP1-87135]

Western Blot: DDX42 Antibody [NBP1-87135] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135] - Staining of human liver shows weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135]

Immunohistochemistry-Paraffin: DDX42 Antibody [NBP1-87135] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Applications for DDX42 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX42

DDX42 encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 42, DEAD box protein 42, EC 3.6.1, EC 3.6.4.13, RHELPSF3b DEAD box protein, RNA helicase-like protein, RNA helicase-related protein, RNAHPFLJ43179, SF3b125 DEAD-box protein, SF3b125ATP-dependent RNA helicase DDX42, Splicing factor 3B-associated 125 kDa protein

Gene Symbol

DDX42

Additional DDX42 Products

Product Documents for DDX42 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DDX42 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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