Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DEC2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human DEC2/SHARP1 inbetween residues 1-75 [UniProt Q9C0J9].

Reactivity Notes

Immunogen has 100% homology to bovine and canine.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit DEC2/SHARP1 Antibody - BSA Free (NBP1-19613) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-DEC2/SHARP1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DEC2/SHARP1 Antibody - BSA Free

Western Blot: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Western Blot: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Huh7 cell lysates loaded from left to right at concentrations of 20, 30, and 40 ug of total protein. Image from verified customer review.
Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody - BSA Free [NBP1-19613]

Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody - BSA Free [NBP1-19613]

Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody [NBP1-19613] - Staining of SHARP1 in mouse pancreas.
Western Blot: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Western Blot: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of SHARP1 in HeLa whole cell extract.
Western Blot: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Western Blot: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of HeLa (1), Raw 264.7 (2), A431 (3), MCF7 (4), and PC12 (5) using SHARP1 antibody at 2 ug/mL.
Simple Western: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Simple Western: DEC2/SHARP1 AntibodyBSA Free [NBP1-19613]

Simple Western: DEC2/SHARP1 Antibody [NBP1-19613] - Image shows a specific band for SHARP1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for DEC2/SHARP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Simple Western

1:50

Western Blot

1 - 2 ug/ml
Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NBP1-19613 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEC2

SHARP1 also known as DEC2 and BHLHe41 encodes a transcription factor that belongs to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors (bHLH). However, sequence alignment shows that SHARP-1 is only distantly related to these proteins, exhibiting 37-42% sequence identity within the bHLH domain. Unlike most bHLH proteins, SHARP-1 is not expressed in neuronal progenitor cells or early differentiating neurons but is restricted to a subset of neurons of the postnatal central nervous system. Recent studies have shown DEC2 plays a regulatory role in clockwork system along with Clock and Bmal1. DEC1, a structurally related protein also known as SHARP2 along with DEC2 works as the negative regulated of molecular clock. Mutations in this gene (hDEC2-P385R) are associated with short sleep phenotypes in human and transgenic mice carrying this mutation show increased vigilance time and less sleep time than control mice.

Long Name

Differentially expressed in chondrocytes protein 2

Alternate Names

BHLHB3, BHLHE41, SHARP-1

Entrez Gene IDs

79365 (Human); 79362 (Mouse); 117095 (Rat)

Gene Symbol

BHLHE41

UniProt

Q9C0J9

Additional DEC2 Products

Product Documents for DEC2/SHARP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DEC2/SHARP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DEC2/SHARP1 Antibody - BSA Free

Customer Reviews for DEC2/SHARP1 Antibody - BSA Free (1)

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Customer Images

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  • DEC2/SHARP1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Huh7 cell lysate
    Species: Human
    Verified Customer | Posted 01/25/2019
    Huh7 cell lysates loaded from left to right with concentrations ( 20, 30, and 40 microgram of total protein
    DEC2/SHARP1 Antibody - BSA Free NBP1-19613

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Protocols

View specific protocols for DEC2/SHARP1 Antibody - BSA Free (NBP1-19613):

DEC2/SHARP1 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.


DEC2/SHARP1 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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FAQs for DEC2/SHARP1 Antibody - BSA Free

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    • Q: Your data sheet shows the sharp-1 MW at around 50KDa. However, when I do Western blot using extract from breast cancer cell lines, I see a band around 35, why the molecular weight of this protein is different on your datasheet?
      A: The expected weight of Sharp-1 is 50 kDa according to UniProt Q9C0J9 (http://www.uniprot.org/uniprot/Q9C0J9) However, if the 35 kDa band you are seeing is consistent with what other companies see, then I would believe that it is the correct band. The 35 kDa band you and other companies are seeing is most likely a degradation product. You may also want to consult the published literature to confirm.
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Associated Pathways

Th2 Differentiation Pathway Th2 Differentiation Pathway Thumbnail