Decorin Antibody (MSVA-537R)
Novus Biologicals | Catalog # NBP3-57256
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # MSVA-537R
Loading...
Product Specifications
Immunogen
Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary) Swissprot P07585
Localization
Secreted
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Fallopian tube: A strong staining should be seen in the stroma while epithelial cells remain negative..
Negative Control
Negitve control:Fallopian tube: Epithelial cells should not show any staining.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Decorin Antibody (MSVA-537R)
Immunohistochemistry-Paraffin:Decorin Antibody (MSVA-537R)NBP3-57256
Serous high grade carcinoma of the ovary showing decorin staining of the stroma
Immunohistochemistry-Paraffin:Decorin Antibody (MSVA-537R)NBP3-57256
Fallopian tube mucosa Strong decorin immunostaining in the stroma while staining is completely lacking in epithelial cells decorin immunohistochemistry.
Immunohistochemistry-Paraffin:Decorin Antibody (MSVA-537R)NBP3-57256
Strong decorin staining of the stroma while urothelium remains unstained in the urinary bladder
Decorin Cancer Tissue Gallery
Decorin Cancer Tissue Gallery
Decorin Cancer Tissue Gallery
Decorin Cancer Tissue Gallery
Decorin Normal Tissue Gallery
Decorin Normal Tissue Gallery
Decorin Normal Tissue Gallery
Decorin Normal Tissue Gallery
Decorin Normal Tissue Gallery
Decorin Normal Tissue Gallery
Applications for Decorin Antibody (MSVA-537R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Decorin
Alternate Names
DCN, DSPG2, PG-II, PGS2, SLRR1B
Gene Symbol
DCN
Additional Decorin Products
Product Documents for Decorin Antibody (MSVA-537R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Decorin Antibody (MSVA-537R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Decorin Antibody (MSVA-537R)
There are currently no reviews for this product. Be the first to review Decorin Antibody (MSVA-537R) and earn rewards!
Have you used Decorin Antibody (MSVA-537R)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
