Delta Np73 Antibody (38C674.2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-33262
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 38C674.2
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was developed against a peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Delta Np73 Antibody (38C674.2) - Azide and BSA Free
![Western Blot: Delta Np73 Antibody (38C674.2)Azide and BSA Free [NBP2-33262]](https://resources.rndsystems.com/images/products/Delta-Np73-Antibody-38C674-2-Azide-Free-Western-Blot-NBP2-33262-img0001.jpg "Western Blot: Delta Np73 Antibody (38C674.2)Azide and BSA Free [NBP2-33262]")
Western Blot: Delta Np73 Antibody (38C674.2)Azide and BSA Free [NBP2-33262]
Western Blot: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) analysis for Np73 using NBP2-24873 at 1 ug/ml in A) a cell line transfected with Np73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]](https://resources.rndsystems.com/images/products/Delta-Np73-Antibody-38C674-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33262-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]")
Immunocytochemistry/ Immunofluorescence: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]
Immunocytochemistry/Immunofluorescence: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Delta Np73 (38C674.2) NBP2-24873 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]](https://resources.rndsystems.com/images/products/Delta-Np73-Antibody-38C674-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33262-img0004.jpg "Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]")
Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]
Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) Formalin-fixed, paraffin-embedded lung squamous cell carcinoma probed with p73 antibody at 5 ug/ml. Image from the standard format of this antibody.![Immunohistochemistry: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]](https://resources.rndsystems.com/images/products/Delta-Np73-Antibody-38C674-2-Azide-Free-Immunohistochemistry-NBP2-33262-img0003.jpg "Immunohistochemistry: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]")
Immunohistochemistry: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]
Immunohistochemistry: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) analysis of Delta Np73 in embryonic day 15 (A) and day 17 (B) E15 rat embryonic kidney at 1:2000. Delta Np73 is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the g![Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]](https://resources.rndsystems.com/images/products/Delta-Np73-Antibody-38C674-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33262-img0005.jpg "Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]")
Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide and BSA Free [NBP2-33262]
Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.Applications for Delta Np73 Antibody (38C674.2) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:1000
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Delta Np73
Alternate Names
p53-related protein, p73, tumor protein p73
Gene Symbol
TP73
Additional Delta Np73 Products
Product Documents for Delta Np73 Antibody (38C674.2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Delta Np73 Antibody (38C674.2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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