DENND1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47486

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DENND1B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AFLCGGSGDQAEWNLGQDDSALHGKHLPPSPRKRVSSSGLTDSLFILKEENSNKHLGADNVSDPTSGLDFQLTSPEVSQTD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DENND1B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DENND1B Antibody [NBP2-47486]

Immunocytochemistry/ Immunofluorescence: DENND1B Antibody [NBP2-47486]

Immunocytochemistry/Immunofluorescence: DENND1B Antibody [NBP2-47486] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
Immunohistochemistry: DENND1B Antibody [NBP2-47486]

Immunohistochemistry: DENND1B Antibody [NBP2-47486]

Immunohistochemistry: DENND1B Antibody [NBP2-47486] - Staining of human colon tissue. Shows strong cytoplasmic positivity in glandular cells.

Applications for DENND1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DENND1B

DENND1B is a member of the connecdenn family, which links RAB35 activation with the clathrin machinery. The protein functions as a guanine nucleotide exchange factor for RAB35. DENND1B is present at higher levels in dendritic and natural killer cells but at lower levels in the other myeloid lineage cells.

Alternate Names

C1ORF18, C1orf218, chromosome 1 open reading frame 218, Connecdenn 2, DENN domain-containing protein 1B, DENN/MADD domain containing 1B, DKFZp547O0715, FAM31BMGC103810, FLJ20054, FLJ21179, FLJ33888, member B, Protein FAM31B

Gene Symbol

DENND1B

Additional DENND1B Products

Product Documents for DENND1B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DENND1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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