DGK-zeta Antibody [Biotin]

Novus Biologicals | Catalog # NBP3-69543

Fc Region Site-Specific Biotinylated Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTPRSLQGDAAPPQGEELIEAAKRNDFCKLQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. Successful site-specific biotin conjugation is confirmed by reducing SDS-PAGE, revealing a mobility shift of the antibody heavy-chain when biotin is covalently attached. Additional information may be available upon request. The antibody concentration is provided on the individual product label. For specialized conjugation needs, like large quantities, and specific concentrations, bulk and custom antibody conjugation services are available.

Applications for DGK-zeta Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
Recommended applications and dilutions are based on validated applications from the unconjugated base product NBP2-13918

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

40% glycerol and PBS (pH 7.2).

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DGK-zeta

DGKZ, also known as Diacylglycerol kinase zeta, consists of six isoforms of sizes 124.1 kDa, 104 kDa, 106 kDa, 104.1 kDa, 104.6 kDa, and 101.2 kDa and is involved in regulating the amount of diacylglycerol in signal transduction pathways as a member of the eukaryotic diacylglycerol kinase family. Current research is exploring the effect of the protein on diseases such as malaria, retinoblastoma, cerebritis, bipolar disorder, and cerebral infarction. The protein interacts with RB1, RBL1, RBL2, MAPK6, and GCOM1 while in glycerolipid metabolism, hemostasis, PIP2 hydrolysis, and signal transduction pathways.

Long Name

Diacylglycerol Kinase

Alternate Names

DGKZ, DGKzeta

Gene Symbol

DGKZ

Additional DGK-zeta Products

Product Documents for DGK-zeta Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DGK-zeta Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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