DHX40 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91834

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVGRTFCTMDGRGSPVHIHPSSALHEQETKLEWIIFHEVLVTTKVYARIVCPIRYEWVRDLLPKLHEFNAHDLSSVARREVREDARRRWTNKENVKQLKDGISKDVLKKMQRRNDDKSISDARARF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX40 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DHX40 Antibody [NBP1-91834]

Immunocytochemistry/ Immunofluorescence: DHX40 Antibody [NBP1-91834]

Immunocytochemistry/Immunofluorescence: DHX40 Antibody [NBP1-91834] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834] - Staining of human endometrium shows weak to moderate nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834] - Staining of human testis shows strong nuclear positivity in Leydig cells, as well as moderate positivity in cells in seminiferous tubules.
Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834] - Staining of human fallopian tube shows weak to moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834]

Immunohistochemistry-Paraffin: DHX40 Antibody [NBP1-91834] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
DHX40 Antibody - BSA Free

Western Blot: DHX40 Antibody - BSA Free [NBP1-91834] -

JMJD6 forms an interaction network that consists of proteins involved in splicing and protein synthesis.(A) FLAG-tagged JMJD6 transduced into SK-N-AS cells for immunoprecipitation with anti-FLAG followed by protein identification by mass spectrometry. The interacting protein partners of JMJD6 are analyzed by STRING protein network. (B) Immunoprecipitation followed by western blot to validate the JMJD6-interacting partners in SK-N-AS cells. IP = immunoprecipitation, FT = flowthrough. n=single experiment. (C) Click-iT AHA labeling showing the newly synthesized proteins after overexpression of JMJD6 in SK-N-AS cells. n=single experiment. (D, E) Western blot showing the expression of GAC and KGA isoforms in SKNAS (D), BE2C (E), after U2AF2 and CPSF6 knockdown for 72 hr. n=single experiment.Figure 6—source data 1.Mass spectrometric analysis JMJD6 interactomes in BE2C and SKNAS cells.Mass spectrometric analysis JMJD6 interactomes in BE2C and SKNAS cells.The JMJD6 interactome in BE2C cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38488852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for DHX40 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHX40

DDX40 is a member of the DExH/D box family of ATP-dependent RNA helicases. RNA helicases catalyze the unwinding of double-stranded RNA and play a role in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, and organellar gene expression.[supplied by OMIM]

Alternate Names

ARG147, DDX40FLJ34015, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 40 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 40, DEAH box protein 40, EC 3.6.1, EC 3.6.4.13, FLJ22060, PAD, probable ATP-dependent RNA helicase DHX40, Protein PAD

Entrez Gene IDs

79665 (Human)

Gene Symbol

DHX40

UniProt

Additional DHX40 Products

Product Documents for DHX40 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DHX40 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DHX40 Antibody - BSA Free

Customer Reviews for DHX40 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review DHX40 Antibody - BSA Free and earn rewards!

Have you used DHX40 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...