DTX3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-31789

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DTX3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKKACPMC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DTX3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DTX3 Antibody [NBP2-31789]

Immunocytochemistry/ Immunofluorescence: DTX3 Antibody [NBP2-31789]

Immunocytochemistry/Immunofluorescence: DTX3 Antibody [NBP2-31789] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus, nucleoli & the Golgi apparatus.
Immunohistochemistry-Paraffin: DTX3 Antibody [NBP2-31789]

Immunohistochemistry-Paraffin: DTX3 Antibody [NBP2-31789]

Immunohistochemistry-Paraffin: DTX3 Antibody [NBP2-31789] - Staining of human stomach, upper shows strong cytoplasmic positivity in parietal cells.

Applications for DTX3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DTX3

DTX3 encodes a probable E3 ubiquitin-protein ligase DTX3 that exists in two isoforms: Isoform 1 id 347 amino acids long at nearly 38 kDA, while isoform 2 is 350 amino acids long at 38 kDA as well. DTX3 is involved in the notch signaling pathway and protein modification and is known to interact with genes UBE2D2, UBE2D3, UBE2L6, UBE2V1, and UBE2W. It is critical in cell to cell communication that affects cell-fate determinations.

Alternate Names

deltex 3 homolog (Drosophila), deltex homolog 3 (Drosophila), Deltex3, FLJ34766, MGC138863, MGC138864, protein deltex-3, RING finger protein 154, RNF154deltex 3 homolog

Gene Symbol

DTX3

UniProt

Q8N9I9 (Human)

Additional DTX3 Products

Product Documents for DTX3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DTX3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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