eIF4GII Antibody

Novus Biologicals | Catalog # NBP2-16309

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all eIF4GII Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF4GII. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

177 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4GII Antibody

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309] - A: Marker. B: mouse testes. 6% SDS-PAGE gel, antibody diluted 1:10000. This image was submitted via customer review.
Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term dilution: 1:500.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term dilution: 1:500.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term dilution: 1:500.
Immunohistochemistry-Frozen: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Frozen: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Frozen: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: eIF4GII protein stained by eIF4GII antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody [N1], N-term detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. eIF4GII stained by eIF4GII diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: eIF4GII Antibody [NBP2-16309]

Immunoprecipitation: eIF4GII Antibody [NBP2-16309]

Immunoprecipitation: eIF4GII Antibody [NBP2-16309] - Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 ug of EIF4G3 antibody [N1], N-term. Western blot analysis was performed using EIF4G3 ntibody [N1], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent
eIF4GII Antibody

Immunocytochemistry/ Immunofluorescence: eIF4GII Antibody [NBP2-16309] -

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (NBP2-16309) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for eIF4GII Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Reviewed Applications

Read 1 review rated 5 using NBP2-16309 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF4GII

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1

Alternate Names

eIF-4G 3, eIF-4-gamma 3, eIF-4-gamma II, eIF4GIIeIF4G 3, eukaryotic translation initiation factor 4 gamma 3, eukaryotic translation initiation factor 4 gamma, 3

Gene Symbol

EIF4G3

UniProt

O43432 (Human)

Additional eIF4GII Products

Product Documents for eIF4GII Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for eIF4GII Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for eIF4GII Antibody

Customer Reviews for eIF4GII Antibody (1)

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Customer Images

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  • eIF4GII Antibody
    Name: LEI ZHANG
    Application: Western Blot
    Sample Tested: testes
    Species: Mouse
    Verified Customer | Posted 04/02/2018
    Western blot: eIF4G II Antibody [NBP2-16309] –Sample (40 ug of whole cell lysate) A: Marker B: mouse testes 6% SDS-PAGE gel, diluted at 1:10000
    eIF4GII Antibody NBP2-16309

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Protocols

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