Elastin Antibody (MSVA-648R)

Novus Biologicals | Catalog # NBP3-57257

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-648R
View all Elastin Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Elastin protein Swissprot P15502

Localization

Extracellular

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial)..

Negative Control
Negitve control:Colon: Elastin immunostaining should be absent in all epithelial cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Applications for Elastin Antibody (MSVA-648R)

Application
Recommended Usage

Immunohistochemistry

1:100 – 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Elastin

Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

Alternate Names

elastin, FLJ38671, FLJ43523, SVAS, tropoelastin, WBS, WS

Gene Symbol

ELN

Additional Elastin Products

Product Documents for Elastin Antibody (MSVA-648R)

Certificate of Analysis

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Product Specific Notices for Elastin Antibody (MSVA-648R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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