ELMO1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33645

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ELMO1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO1. The exact sequence is proprietary.

Localization

Cytoplasm, Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ELMO1 Antibody - BSA Free

Western Blot: ELMO1 Antibody [NBP1-33645]

Western Blot: ELMO1 Antibody [NBP1-33645]

Western Blot: ELMO1 Antibody [NBP1-33645] - ELMO1 antibody detects ELMO1 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 7.5% SDS-PAGE. ELMO1 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ELMO1 Antibody [NBP1-33645]

Immunocytochemistry/ Immunofluorescence: ELMO1 Antibody [NBP1-33645]

Immunocytochemistry/Immunofluorescence: ELMO1 Antibody [NBP1-33645] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ELMO1 Antibody [NBP1-33645]

Immunohistochemistry-Paraffin: ELMO1 Antibody [NBP1-33645]

Immunohistochemistry-Paraffin: ELMO1 Antibody [NBP1-33645] - Human stomach, using ELMO1antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: ELMO1 Antibody [NBP1-33645]

Western Blot: ELMO1 Antibody [NBP1-33645]

Western Blot: ELMO1 Antibody [NBP1-33645] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: ELMO1 Antibody [NBP1-33645]

Western Blot: ELMO1 Antibody [NBP1-33645]

Western Blot: ELMO1 Antibody [NBP1-33645] - ELMO1 antibody detects ELMO1 protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. ELMO1 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ELMO1 Antibody ELMO1 Antibody

Western Blot: ELMO1 Antibody [NBP1-33645] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE diluted at 1:1000.

Western Blot: ELMO1 Antibody [NBP1-33645] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE diluted at 1:1000.

Applications for ELMO1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ELMO1

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Alternate Names

ced-12 homolog 1, CED12CED-12, ELMO-1, engulfment and cell motility 1, engulfment and cell motility 1 (ced-12 homolog, C. elegans), engulfment and cell motility protein 1, KIAA0281MGC126406, Protein ced-12 homolog

Gene Symbol

ELMO1

Additional ELMO1 Products

Product Documents for ELMO1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ELMO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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