Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 6E1

Format

Azide and BSA Free
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Product Specifications

Immunogen

Mouse Recombinant EphA7 (extracellular domain)

Specificity

This was selected for its ability to detect mouse EphA7 in western blots. In this format, this does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA8 and EphB2, EphB3 or EphB4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Description

Novus Biologicals Rat EphA7 Antibody (6E1) - Azide and BSA Free (NB110-93606) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EphA7 Antibody (6E1) - Azide and BSA Free

Immunohistochemistry-Paraffin: EphA7 Antibody (6E1) - Azide and BSA Free [NB110-93606]

Immunohistochemistry-Paraffin: EphA7 Antibody (6E1) - Azide and BSA Free [NB110-93606]

Immunohistochemistry-Paraffin: EphA7 Antibody (6E1) [NB110-93606] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NB110-93606

Applications for EphA7 Antibody (6E1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA7

The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8(Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5(Hek9) and EphB6 (Mep). EphAs are a family of receptor tyrosine kinases that are involved in axonal guidance during development. These receptors and their ligands, the ephrins, act via repulsive mechanisms to guide growing axons towards their appropriate targets and allow for the correct developmental connections to be made. Ligand binding to an Eph receptor results in tyrosine phosphorylation of the kinase domain, and repulsion of axonal growth cones and migrating cells. During neurulation, ephrin-A5 is coexpressed with its cognate receptor EphA7 in cells at the edges of the dorsal neural folds. Three different EphA7 splice variants, a full-length form and two truncated versions lacking kinase domains, are expressed in the neural folds.

Long Name

Eph Receptor A7

Alternate Names

Cek11, Ebk, Ehk3, Hek11, Mdk1

Entrez Gene IDs

2045 (Human); 13841 (Mouse)

Gene Symbol

EPHA7

Additional EphA7 Products

Product Documents for EphA7 Antibody (6E1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EphA7 Antibody (6E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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