ErbB2/Her2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84584

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTA

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ErbB2/Her2 Antibody - BSA Free (NBP1-84584) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ErbB2/Her2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ErbB2/Her2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows no positivity in tumor cells as expected.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows strong membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human kidney shows weak to moderate granular cytoplasmic positivity in cells in tubules.
ErbB2/Her2 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody - BSA Free [NBP1-84584]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody - BSA Free [NBP1-84584]

Staining of human cell line MCF7 shows localization to plasma membrane.
ErbB2/Her2 Antibody - BSA Free Western Blot: ErbB2/Her2 Antibody - BSA Free [NBP1-84584]

Western Blot: ErbB2/Her2 Antibody - BSA Free [NBP1-84584]

Analysis in human cell line SK-BR-3.

Applications for ErbB2/Her2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ErbB2/Her2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ErbB2/Her2 Antibody - BSA Free

Customer Reviews for ErbB2/Her2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ErbB2/Her2 Antibody - BSA Free and earn rewards!

Have you used ErbB2/Her2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody - BSA Free

Showing  1 - 1 的 1 FAQ Showing All

    • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
      A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
Showing  1 - 1 的 1 FAQ Showing All
View all FAQs for Antibodies