ERp57/PDIA3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-78614

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ERp57/PDIA3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERp57/PDIA3. The exact sequence is proprietary.

Localization

Cell membrane

Marker

Endoplasmic Reticulum Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERp57/PDIA3 Antibody - BSA Free

Western Blot: ERp57/PDIA3 Antibody [NB100-78614]

Western Blot: ERp57/PDIA3 Antibody [NB100-78614]

Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Paraffin-embedded mouse lymph node. ERp57 antibody [C3], C-term dilution: 1:500.
Western Blot: ERp57/PDIA3 Antibody [NB100-78614]

Western Blot: ERp57/PDIA3 Antibody [NB100-78614]

Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:1000
Western Blot: ERp57/PDIA3 Antibody [NB100-78614]

Western Blot: ERp57/PDIA3 Antibody [NB100-78614]

Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - Sample: MCF-7 cells were fixed in iced-cold MeOH for 5 min. Green: PDIA3 protein stained by ERp57 antibody, C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - H1299 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Mouse intestine. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Rat middle brain. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for ERp57/PDIA3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERp57/PDIA3

Protein Disulfide Isomerase A3(PDIA3) was also names as GRP58, which was first identified by Bourdi et al. (1995), Koivunen et al. (1996), and Hirano et al. (1995). PDIA3 was coded by 505 aa with disulfide isomerase homology. PIDA3 was localizated in nuclear and ER with specific sequence. PDIA3 was mojorily expressed in liver, placenta, and lung, and lower expressed in all other tissues tested. PDIA4 had protein disulfide isomerase activity, thiol-dependent reductase activity, and induced by oncogenic transformation (Koivunen et al., 1996; Hirano et al., 1995)

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Entrez Gene IDs

2923 (Human)

Gene Symbol

PDIA3

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERp57/PDIA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

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Protocols

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