ERp57/PDIA3 Antibody

Novus Biologicals | Catalog # NBP2-16369

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%), Chimpanzee (100%), Porcine (94%), Rabbit (94%), Rhesus Macaque (98%), Sheep (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ERp57/PDIA3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERp57/PDIA3. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%).

Localization

Endoplasmic reticulum lumen (By similarity); Melanosome

Marker

Endoplasmic Reticulum Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERp57/PDIA3 Antibody

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - Mouse testis. ERp57 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted at 1:500.
Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369]

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted by 1:10000.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - U87 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
ERp57/PDIA3 Antibody

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody [NBP2-16369] -

ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody (NBP2-16369) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.

Applications for ERp57/PDIA3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERp57/PDIA3

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Gene Symbol

PDIA3

UniProt

P30101

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERp57/PDIA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

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Protocols

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FAQs

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