FBXL2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33641

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FBXL2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human FBXL2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBXL2 Antibody - BSA Free

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.
FBXL2 Antibody

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NBP1-33641] -

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NBP1-33641] - FBXL2 antibody [N1], N-term detects FBXL2 protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FBXL2 stained by FBXL2 antibody [N1], N-term (NBP1-33641) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
FBXL2 Antibody

Western Blot: FBXL2 Antibody [NBP1-33641] -

Western Blot: FBXL2 Antibody [NBP1-33641] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FBXL2 antibody [N1], N-term (NBP1-33641) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FBXL2 Antibody

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641] -

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641] - FBXL2 antibody [N1], N-term detects FBXL2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FBXL2 stained by FBXL2 antibody [N1], N-term (NBP1-33641) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for FBXL2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FBXL2

FBXL2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.

Alternate Names

FBL2FBL3F-box protein containing leucine-rich repeats, F-box and leucine-rich repeat protein 2DKFZp564P0622, F-box protein FBL2/FBL3, F-box/LRR-repeat protein 2

Gene Symbol

FBXL2

Additional FBXL2 Products

Product Documents for FBXL2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FBXL2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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