FRMD3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33879

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FRMD3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VVEASSKIQREPPEVHRANITQSRSSHSLNKQLIINMEPLQPLLPSPSEQEEELPLGEGVPLPKEENISAPLISSSPVKAAREYEDPPSEEEDKIKEEPLTISELVYNPSASLLPTPVDDDEIDMLFDCPSRLELEREDTDSFEDLEADE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FRMD3 Antibody - BSA Free

Western Blot: FRMD3 Antibody [NBP2-33879]

Western Blot: FRMD3 Antibody [NBP2-33879]

Western Blot: FRMD3 Antibody [NBP2-33879] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-223
Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and glomeruli.
Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879] - Staining of human ovary shows strong membranous and cytoplasmic positivity in follicle cells.
Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879]

Immunohistochemistry-Paraffin: FRMD3 Antibody [NBP2-33879] - Staining of human tonsil shows moderate membranous positivity in non-germinal center cells.
FRMD3 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: FRMD3 Antibody [NBP2-33879]

Immunocytochemistry/ Immunofluorescence: FRMD3 Antibody [NBP2-33879]

Staining of human cell line SH-SY5Y shows localization to nucleoplasm & plasma membrane.

Applications for FRMD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FRMD3

Protein 4.1 of red blood cells, or 4.1R (EPB41; MIM 130500), is a multifunctional protein essential for maintaining erythrocyte shape and membrane mechanical properties. The protein 4.1 family comprises a group of structural proteins that includes, in addition to 4.1R, 4.1G (general type; MIM 603237), 4.1B (brain type; MIM 605331), 4.1N (neuron type; MIM 602879), and 4.1O (ovary type).[supplied by OMIM]

Alternate Names

band 4.1-like protein 4, Band 4.1-like protein 4O, EPB41L4OMGC20553, EPB41LO, FERM domain containing 3, FERM domain-containing protein 3,4.1O, Ovary type protein 4.1, P410, protein 4.1O

Gene Symbol

FRMD3

UniProt

A2A2Y4 (Human)

Additional FRMD3 Products

Product Documents for FRMD3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FRMD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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