G3BP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16563

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (95%), Porcine (94%), Rat (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G3BP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human G3BP1. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%).

Localization

Cytoplasm; cytosol; Cell membrane; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G3BP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody [NBP2-16563]

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody [NBP2-16563]

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody [NBP2-16563] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G3BP1 stained by G3BP1 antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
G3BP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: G3BP1 Antibody [NBP2-16563] -

G3BP1 antibody detects G3BP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human testis.
G3BP1 stained by G3BP1 antibody (NBP2-16563) diluted at 1:250.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
G3BP1 Antibody - BSA Free

Western Blot: G3BP1 Antibody [NBP2-16563] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody (NBP2-16563) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for G3BP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in PC12 cells, lysates

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: G3BP1

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Gene Symbol

G3BP1

Additional G3BP1 Products

Product Documents for G3BP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for G3BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for G3BP1 Antibody - BSA Free

Customer Reviews for G3BP1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review G3BP1 Antibody - BSA Free and earn rewards!

Have you used G3BP1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies