GABA-A-R delta N-Terminus Antibody

Name: GABA-A-R delta N-Terminus Antibody
Brand: R&D Systems
SKU: PPS090

R&D Systems | Catalog # PPS090

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Product Documents

Discontinued Product

PPS090 has been discontinued. View all GABA-A R delta products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all GABA-A R delta Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytosolic loop of the rat GABA_A R delta subunit, N-Terminus

Specificity

Human, mouse, rat, and bovine ~52 kDa GABAA R delta subunit, N-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GABA-A-R delta N-Terminus Antibody

Detection of GABA_AR delta N-Terminus by Western Blot.

Western blot of mouse cerebellar lysates from wild type (Control) and delta knockout (KO) animals showing specific immunolabeling of the ~52 kDa delta subunit of the GABA_AR. The labeling was absent from a lysate prepared from knockout animals.

Applications for GABA-A-R delta N-Terminus Antibody

Application

Recommended Usage

Immunohistochemistry

1:250 dilution
Sample: (frozen sections; unpublished observations)

Immunoprecipitation

10 μL/50 μg cell lysate

Western Blot

1:1000 dilution
Sample: Mouse brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R delta

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. Receptors in this group operate as GABA-gated Cl- channels. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Typical GABA_A receptors have some combination of an alpha, beta, and gamma subunit. In select neurons, however, a delta subunit replaces the gamma -subunit. It would appear that delta subunits have a preference for various alpha -beta combinations. In cerebellar granule cells, the delta subunit contributes to a unique alpha 6 beta delta heteromer. delta subunit receptors may function to limit the spread of excitatory impulses to dendritically-complexed neurons. They also appear to be sensitive to steroid modulation. The rat delta subunit is a 50 kDa, 433 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 4 - 246). The rat and mouse N-terminal extracellular domains (ECD) (aa 17 - 248) are 99% aa identical; the mouse and human ECD are 95% aa identical.

References

Whiting, P.J. et al. (1999) Ann. N.Y. Acad. Sci. 868:645.
Rudolph, U. et al. (2001) Trends Pharmacol. Sci. 22:188.
Shivers, B.D. et al. (1989) Neuron 3:327.
Sommer, B. et al. (1990) DNA Cell Biol. 9:561.
Bianchi, M.T. and R.L. MacDonald (2003) J. Neurosci. 23:10934.

Long Name

gamma-Aminobutyric Acid Type A Receptor, delta Polypeptide

Alternate Names

GABAAR delta, GABAARd, GABRD

Entrez Gene IDs

2563 (Human); 14403 (Mouse); 29689 (Rat)

Gene Symbol

GABRD

Additional GABA-A R delta Products

Product Documents for GABA-A-R delta N-Terminus Antibody

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Certificate of Analysis

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Product Specific Notices for GABA-A-R delta N-Terminus Antibody

For research use only

Related Research Areas

GABA Receptors

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