GAD65/67 Antibody

Novus Biologicals | Catalog # NBP2-80511

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all GAD65/67 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 530-600 of mouse GAD65 conjugated to blue carrier protein was used as the antigen. The peptide is identical in GAD67. The antigen is homologous in rat and human.

Reactivity Notes

Other species not yet tested but expected to work in zebrafish, canis familiaris, macaca mulatta, sus scrofa.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for GAD65/67 Antibody

Western Blot: GAD65/67 Antibody [NBP2-80511]

Western Blot: GAD65/67 Antibody [NBP2-80511]

Western Blot: GAD65/67 Antibody [NBP2-80511] - Rat brain lysate using Rabbit antibody to GAD65, at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20
Immunohistochemistry-Paraffin: GAD65/67 Antibody [NBP2-80511]

Immunohistochemistry-Paraffin: GAD65/67 Antibody [NBP2-80511]

Immunohistochemistry-Paraffin: GAD65/67 Antibody [NBP2-80511] - Mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
Immunohistochemistry-Paraffin: GAD65/67 Antibody [NBP2-80511]

Immunohistochemistry-Paraffin: GAD65/67 Antibody [NBP2-80511]

Immunohistochemistry-Paraffin: GAD65/67 Antibody [NBP2-80511] - Sections of mouse cerebellum. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.

Applications for GAD65/67 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000 - 1:2000

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Formulation

Lyophilized from whole serum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAD65/67

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Alternate Names

67kD), EC 4.1.1, FLJ45882, glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 67 kDa isoform

Gene Symbol

GAD1

Additional GAD65/67 Products

Product Documents for GAD65/67 Antibody

Certificate of Analysis

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Product Specific Notices for GAD65/67 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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