Galectin-1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-16580
![Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16580]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16580-img0016.jpg "Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16580]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Galectin-1. The exact sequence is proprietary.
Reactivity Notes
Bovine (85%), Dog (81%), Pig (84%), Sheep (84%).
Localization
Secreted; extracellular space; extracellular matrix
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Galectin-1 Antibody - BSA Free (NBP2-16580) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Galectin-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16580]
Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16580] - Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 1 stained by Galectin 1 antibody diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10 um.![Western Blot: Galectin-1 Antibody [NBP2-16580]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0001.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580]")
Western Blot: Galectin-1 Antibody [NBP2-16580]
Western Blot: Galectin 1 Antibody [NBP2-16580] - Sample (30 ug of whole cell lysate) A: BCL-1 15% SDS PAGE gel, diluted at 1:10000.![Western Blot: Galectin-1 Antibody [NBP2-16580]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0002.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580]")
Western Blot: Galectin-1 Antibody [NBP2-16580]
Western Blot: Galectin 1 Antibody [NBP2-16580] - Sample (30 ug of whole cell lysate) A: NCI-H929 15% SDS PAGE gel, diluted at 1:10000.![Western Blot: Galectin-1 Antibody [NBP2-16580]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0014.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580]")
Western Blot: Galectin-1 Antibody [NBP2-16580]
Western Blot: Galectin-1 Antibody [NBP2-16580] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP2-16580]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16580-img0003.jpg "Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP2-16580]")
Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP2-16580]
Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP2-16580] - Human colon carcinoma, using Galectin 1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Frozen: Galectin-1 Antibody [NBP2-16580] -
Galectin 1 antibody detects Galectin 1 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain.
Green: Galectin 1 protein stained by Galectin 1 antibody (NBP2-16580) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: Galectin-1 Antibody [NBP2-16580] -
Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (NBP2-16580) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Galectin-1 Antibody [NBP2-16580] -
Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (NBP2-16580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Galectin-1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
Assay dependent
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Galectin-1
Additional Galectin-1 Products
Product Documents for Galectin-1 Antibody - BSA Free
Product Specific Notices for Galectin-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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