GDI2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03650

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GDI2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2). QVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GDI2 Antibody - BSA Free

Western Blot: GDI2 AntibodyBSA Free [NBP3-03650]

Western Blot: GDI2 AntibodyBSA Free [NBP3-03650]

Western Blot: GDI2 Antibody [NBP3-03650] - Analysis of extracts of various cell lines, using GDI2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Knockout Validated: GDI2 Antibody - BSA Free [NBP3-03650]

Western Blot: GDI2 Antibody - BSA Free [NBP3-03650]

Western Blot: GDI2 Antibody [NBP3-03650] - Analysis of extracts from normal (control) and GDI2 knockout (KO) 293T cells, using GDI2 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
GDI2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GDI2 Antibody - BSA Free [NBP3-03650] -

Immunocytochemistry/ Immunofluorescence: GDI2 Antibody - BSA Free [NBP3-03650] - Immunofluorescence analysis of NIH/3T3 cells using GDI2 antibody (A8615) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for GDI2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:200-1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GDI2

GDP dissociation inhibitors like GDI2 are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements.

Alternate Names

beta, FLJ16452, GDP dissociation inhibitor 2, Guanosine diphosphate dissociation inhibitor 2, RABGDIBFLJ37352

Gene Symbol

GDI2

Additional GDI2 Products

Product Documents for GDI2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GDI2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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