GFAP Antibody

Novus Biologicals | Catalog # NB110-93480

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Equine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

This GFAP Antibody was developed against recombinant and purified bovine GFAP. Accession # Q28115

Reactivity Notes

It is expected that the antibody will react with other mammalian tissues.

Marker

Astrocyte Marker

Specificity

Specific for the 50 kDa GFAP protein. A lower band at 45 kDa is a proteolytic fragment derived from the GFAP molecule.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for GFAP Antibody

Western Blot: GFAP AntibodyAzide and BSA Free [NB110-93480]

Western Blot: GFAP AntibodyAzide and BSA Free [NB110-93480]

Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).
Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Showing specific labeling of GFAP (red) and vimentin (green) cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000, green), colabeled with anti-MeCP2 (1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000,green), colabeled with anti-calbindin (1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody - Azide and BSA Free [NB110-93480]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).

Applications for GFAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:300 - 1:5000

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Neat whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GFAP

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

Q28115 (Bovine)

Additional GFAP Products

Product Documents for GFAP Antibody

Certificate of Analysis

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Product Specific Notices for GFAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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