GLUT9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-05054

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLUT9 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human GLUT9 (within residues 500-540). [Swiss-Prot# Q9NRM0]

Localization

Membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLUT9 Antibody - BSA Free

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054]

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054]

GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0006.jpg
Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054]

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054]

GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0007.jpg
Western Blot: GLUT9 AntibodyBSA Free [NBP1-05054]

Western Blot: GLUT9 AntibodyBSA Free [NBP1-05054]

Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.
Immunocytochemistry/ Immunofluorescence: GLUT9 Antibody - BSA Free [NBP1-05054]

Immunocytochemistry/ Immunofluorescence: GLUT9 Antibody - BSA Free [NBP1-05054]

Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: GLUT9 Antibody - BSA Free [NBP1-05054]

Immunohistochemistry-Paraffin: GLUT9 Antibody - BSA Free [NBP1-05054]

Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.
GLUT9 Antibody - BSA Free

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] -

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody & b antigen-preabsorbed antibody. Scale bar 100 µm. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) & DAPI (blue) on ependymal cells. Scale bar 10 µm. e–g Immunofluorescence staining of GLUT9 (magenta) & NeuN (green) showing co-localization in neurons. Scale bar 10 µm. D3V, dorsal third ventricle; DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nucleus marker Image collected & cropped by CiteAb from the following publication (https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLUT9 Antibody - BSA Free

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] -

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of ABCG2 in methanol/acetone-fixed & methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) & ABCG2 knockout (KO) mice & post-fixed with methanol & acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus & capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) & GLUT9 (green) showing ABCG2 in the choroid plexus & GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 & e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle Image collected & cropped by CiteAb from the following publication (https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GLUT9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

0.5 ug/ml
Application Notes
This GLUT9 antibody is useful for Western blot, where a band is seen ~ 55 kDa. The immunogen of this product corresponds to a cytoplasmic domain of GLUT9 protein and in immunostaining assays, cytoplasmic-membrane staining may be expected for this product. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP1-05054 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLUT9

GLUT9 (glucose transporter 9; SLC2A9) is a multi-pass membrane transporter protein which primarily transport urate and fructose, and may also implicate in urate reabsorption by proximal tubules as well as the transportation of glucose at low rate. GLUT9 belongs to sugar transporter (TC 2.A.1.1) family and SLC2A9 gene encodes two transcripts: a long isoform (GLUT9L/SLC2A9L) and a short isoform (GLUT9S/SLC2A9S). Placenta is one of the few tissues that express both variants suggesting their role in placental hexose transport, and GLUT9 has been shown to transport both glucose and fructose with about 3-fold higher affinity for glucose. GLUT9 has emerged as a high-capacity urate transporter which influences serum uric acid levels; and GLUT9L and GLUT9S isoforms shows strong expression in the basolateral and apical membranes, respectively, of proximal renal tubular cells. GLUT9 is an important modulator responsible for the reabsorption of urate in the apical membrane of the renal proximal tubules, and loss-of-function mutations in GLUT9 gene results in functional impairment associated with renal hypouricemia type 2 (RHUC2), a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Alternate Names

facilitated glucose transporter member 9, GLUT-9, GLUT9Glut9, GLUTX, solute carrier family 2 (facilitated glucose transporter), member 9, UAQTL2, urate voltage-driven efflux transporter 1

Gene Symbol

SLC2A9

Additional GLUT9 Products

Product Documents for GLUT9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLUT9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLUT9 Antibody - BSA Free

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  • GLUT9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Goose Tissue
    Species: Goose
    Verified Customer | Posted 09/22/2017
    GLUT9 Antibody - BSA Free NBP1-05054

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Protocols

View specific protocols for GLUT9 Antibody - BSA Free (NBP1-05054):

GLUT9 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 30 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-GLUT9 primary antibody (NBP1-05054) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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