Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free
Novus Biologicals | Catalog # NBP3-12166
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Description
Store at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use
Scientific Data Images for Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free
Western Blot: Glutathione S-Transferase/GST Antibody (GST13)VHH [NBP3-12166]
Western Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Recombinant GST (0.2ug) [+]. Lane 3: HEK293T WCL / rec. GST(10ug/0.2ug) [+]. Lane 4: HEK293T Whole Cell Lysate (10ug) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (10ug) [-]. Primary Antibody: Anti-GST (VhH) at 5.0ug/mL overnight at 2-8 degrees C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:3,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 60 sec. Predicted MW: ~25.5kDa. Observed MW: ~25.5kDa. Unspecific Bands: ~70kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.Applications for Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free
Dot Blot
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
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Background: Glutathione S-Transferase/GST
Alternate Names
Additional Glutathione S-Transferase/GST Products
Product Documents for Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free
Certificate of Analysis
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Product Specific Notices for Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars