Glutathione S Transferase kappa 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89726

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glutathione S Transferase kappa 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione S Transferase kappa 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP1-89726]

Immunocytochemistry/ Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP1-89726]

Immunocytochemistry/Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP1-89726] - Staining of human cell line A-431 shows localization to peroxisomes.
Glutathione S Transferase kappa 1 Antibody - BSA Free Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Glutathione S Transferase kappa 1 Antibody - BSA Free Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Glutathione S Transferase kappa 1 Antibody - BSA Free Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Glutathione S Transferase kappa 1 Antibody - BSA Free Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody - BSA Free [NBP1-89726]

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Applications for Glutathione S Transferase kappa 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione S Transferase kappa 1

Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells (1) The Kappa class of GSTs comprises soluble enzymes originally isolated from the mitochondrial matrix of rats. Gsk1 catalyses some typical GST reactions, it has been proposed that it is structurally distinct from other classes of cytosolic GSTs (2). Gsk1 has been identified in rat, mouse, and human. The C terminus of Gsk1 was essential for localization of the protein to peroxisomes, and the C-terminal sequence Ala-Arg-Leu represents a peroxisome targeting signal (3).

Alternate Names

EC 2.5.1.18, glutathione S-transferase k1, glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, glutathione S-transferase subunit 13 homolog, GST, GST 13-13, GST class-kappa, GST13, GST13-13, GSTK1-1, hGSTK1

Entrez Gene IDs

373156 (Human)

Gene Symbol

GSTK1

UniProt

Q9Y2Q3 (Human)

Additional Glutathione S Transferase kappa 1 Products

Product Documents for Glutathione S Transferase kappa 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glutathione S Transferase kappa 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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