Glypican 1 Antibody (S03-2E8)

Novus Biologicals | Catalog # NBP3-19539

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-2E8 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide of human Glypican 1/GPC1 (Uniprot # P35052)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glypican 1 Antibody (S03-2E8)

Western Blot: Glypican 1 Antibody (S03-2E8) [NBP3-19539]

Western Blot: Glypican 1 Antibody (S03-2E8) [NBP3-19539]

Western Blot: Glypican 1 Antibody (S03-2E8) [NBP3-19539] - Western blot detection of Glypican 1 in Jurkat, Hela cell lysates using NBP3-19539 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 55kDa.
Glypican 1 Antibody (S03-2E8)

Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody (S03-2E8) [NBP3-19539] -

Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody (S03-2E8) [NBP3-19539] - Immunofluorescence of Glypican 1 (green) in Hela using NBP3-19539 at dilution 1/20, and DAPI (blue)

Applications for Glypican 1 Antibody (S03-2E8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/100

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 1

GPC1(Glypican 1) is a cell surface proteoglycan that contains heparan sulphate. The protein is attached to the cell membrane by a GPI anchor. GPC1 is required for efficient TGF-beta1 signaling in pancreatic cancer cells. Members of the glypican-related integral membrane proteoglycan family (GRIPS) may play a role in the control of cell division and growth regulation.

Alternate Names

GPC1

Gene Symbol

GPC1

Additional Glypican 1 Products

Product Documents for Glypican 1 Antibody (S03-2E8)

Certificate of Analysis

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Product Specific Notices for Glypican 1 Antibody (S03-2E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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