GNB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16699

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (100%), Canine (100%), Chicken (96%), Guinea Pig (100%), Rhesus Macaque (100%), Xenopus (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GNB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (96%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GNB1 Antibody - BSA Free

Western Blot: GNB1 Antibody [NBP2-16699]

Western Blot: GNB1 Antibody [NBP2-16699]

Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699]

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699]

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Mouse testis. GNB1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699]

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699]

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Human lung papillory adenocarcinoma, using GNB1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation: GNB1 Antibody [NBP2-16699]

Immunoprecipitation: GNB1 Antibody [NBP2-16699]

Immunoprecipitation: GNB1 Antibody [NBP2-16699] - Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 ug of GNB1 antibody. Western blot analysis was performed using GNB1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
GNB1 Antibody

Immunohistochemistry Whole-Mount:GNB1 Antibody [NBP2-16699] -

Immunohistochemistry Whole-Mount:GNB1 Antibody [NBP2-16699] -Analysis (whole mount) of zebrafish embryo, using GNB1 antibody at 1:200 dilution.
GNB1 Antibody - BSA Free

Western Blot: GNB1 Antibody [NBP2-16699] -

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (NBP2-16699) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GNB1 Antibody - BSA Free

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] -

GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GNB1 antibody (NBP2-16699) diluted at 1:1000.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GNB1 Antibody - BSA Free

Western Blot: GNB1 Antibody [NBP2-16699] -

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (NBP2-16699) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GNB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GNB1 Antibody [NBP2-16699] -

GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNB1 protein stained by GNB1 antibody (NBP2-16699) diluted at 1:500.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000.
Blue: Hoechst 33342 staining.
GNB1 Antibody - BSA Free

Western Blot: GNB1 Antibody [NBP2-16699] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (NBP2-16699) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GNB1 Antibody - BSA Free

Immunohistochemistry: GNB1 Antibody [NBP2-16699] -

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNB1 antibody (NBP2-16699) at 1:300 dilution.

Applications for GNB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Proximity Ligation Assay

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GNB1

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]

Alternate Names

beta subunit, signal-transducing proteins GS/GI, G protein, beta-1 subunit, guanine nucleotide binding protein (G protein), beta polypeptide 1, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1

Gene Symbol

GNB1

Additional GNB1 Products

Product Documents for GNB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GNB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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