GP2 Antibody (MSVA-475M) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57327

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # MSVA-475M

Format

Azide and BSA Free
View all GP2/ZAP75 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary) Swissprot P55259

Localization

Cytoplasmic and cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity.

Negative Control
Negitve control:Colon: GP2 staining should be completely absent in glandular and stromal cells.

Applications for GP2 Antibody (MSVA-475M) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GP2/ZAP75

Long Name

Glycoprotein 2

Alternate Names

GP2, ZAP75

Gene Symbol

GP2

Additional GP2/ZAP75 Products

Product Documents for GP2 Antibody (MSVA-475M) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GP2 Antibody (MSVA-475M) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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