![GPR133 Antibody Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]](https://resources.rndsystems.com/images/products/nbp1-85142_-immunohistochemistry-639204443915406335.jpg "Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (91%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NSEVRAAFKHKTKVWSLTSSSARTSNAKPFHSDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV
Reactivity Notes
Rat (86%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GPR133 Antibody
Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]
Staining of human prostate shows moderate membranous and cytoplasmic positivity in glandular cells.![GPR133 Antibody Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]](https://resources.rndsystems.com/images/products/nbp1-85142_-immunohistochemistry-639204443916006857.jpg "Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]")
Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]
Staining of human urinary bladder shows moderate membranous positivity in urothelial cells.![GPR133 Antibody Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]](https://resources.rndsystems.com/images/products/nbp1-85142_-immunohistochemistry-639204443920422785.jpg "Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]")
Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]
Staining of human placenta shows weak membranous positivity in trophoblastic cells.![GPR133 Antibody Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]](https://resources.rndsystems.com/images/products/nbp1-85142_-immunohistochemistry-639204443941596207.jpg "Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]")
Immunohistochemistry: GPR133 Antibody [GPR133 Antibody]
Staining of human liver shows no positivity in hepatocytes as expected.Applications for GPR133 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GPR133
Alternate Names
PGR25
Gene Symbol
ADGRD1
Additional GPR133 Products
Product Documents for GPR133 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPR133 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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