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Key Product Details
Species Reactivity
Validated:
Human, Rat, Bat, Canine, Hamster, Monkey, Primate, Rabbit
Predicted:
Equine (94%), Mouse (94%), Porcine (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 16 amino acid peptide from 2nd extracellular domain of human GPR81.
Epitope
2nd extracellular domain
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Bovine (88%).
Localization
Multi-pass membrane protein
Specificity
Human GPR81. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for GPR81 Antibody - BSA Free
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]
Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.![Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]](https://beta-resources.rndsystems.com/images/products/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0001.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]")
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]
Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Analysis of anti-FKSG80 / GPR81 antibody with pituitary, anterior at 3 ug/ ml.![Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]](https://beta-resources.rndsystems.com/images/products/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0002.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]")
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]
Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Analysis of anti-FKSG80 / GPR81 antibody with human brain, glioblastoma.![Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]](https://beta-resources.rndsystems.com/images/products/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0003.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]")
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]
Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human pancreas islet of Langerhans. Imunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.![Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]](https://beta-resources.rndsystems.com/images/products/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0004.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]")
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2091]
Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.Applications for GPR81 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
3 ug/ml
Application Notes
.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: GPR81
Long Name
G Protein-coupled Receptor 81
Alternate Names
FKSG80, GPR104, HCA1, HCAR1, LACR1, TA-GPCR
Entrez Gene IDs
27198 (Human)
Gene Symbol
HCAR1
UniProt
Additional GPR81 Products
Product Documents for GPR81 Antibody - BSA Free
Product Specific Notices for GPR81 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
View specific protocols for GPR81 Antibody - BSA Free (NLS2091):
Immunohistochemistry Protocol for GPR81 Antibody (NLS2091):
Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.
**Do not allow the tissues to dry at any time during the staining procedure**
12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.
**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.
Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.
**Do not allow the tissues to dry at any time during the staining procedure**
12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.
**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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