Granzyme B Antibody

Novus Biologicals | Catalog # NB100-684

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Granzyme B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: EIIGGHEAKPHSRPYMAYL, corresponding to amino acids 20-38 of Human Granzyme B (N-terminus).

Localization

Cytoplasmic

Specificity

This reacts with a 32 kDa protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granzyme B Antibody

Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody [NB100-684]

Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody [NB100-684]

Immunocytochemistry/Immunofluorescence: Granzyme B Antibody [NB100-684] - Staining of Granzyme B in red on FFPE human Tonsil. Secondary antibody is donkey anti-rabbit A647. Nuclei counter-stained with DAPI. Staining pattern is cytoplasmic with obvious polarization (see arrowheads) in some cases. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Granzyme B Antibody [NB100-684]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NB100-684]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NB100-684] - FFPE human tonsil stained with Granzyme B antibody.

Applications for Granzyme B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:100
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reviewed Applications

Read 1 review rated 5 using NB100-684 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Granzyme B

The lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Entrez Gene IDs

3002 (Human); 14939 (Mouse); 171528 (Rat)

Gene Symbol

GZMB

Additional Granzyme B Products

Product Documents for Granzyme B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Granzyme B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Granzyme B Antibody

Customer Reviews for Granzyme B Antibody (1)

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Customer Images

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  • Granzyme B Antibody
    Name: Manuel Flores
    Application: Immunocytochemistry
    Sample Tested: Human Tonsil tissue
    Species: Human
    Verified Customer | Posted 06/11/2017
    Staining of Granzyme B in red on FFPE human Tonsil. 1ry Ab is NB100-684 from Novus Biologicals. 2ry Ab is donkey anti-rabbit A647. Nuclei countestained with DAPI. Staining pattern is cytoplasmic with obvious polarization (see arrowheads) in some cases.
    Granzyme B Antibody NB100-684

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Protocols

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