GRIM19 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90050

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRIM19 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IMKWNRERRRLQIEDFEARIALLPLLQAETDRRTLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVPPLIGELYGLRTTEEALHASHGFMWYT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRIM19 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRIM19 Antibody [NBP1-90050]

Immunocytochemistry/ Immunofluorescence: GRIM19 Antibody [NBP1-90050]

Immunocytochemistry/Immunofluorescence: GRIM19 Antibody [NBP1-90050] - Staining of human cell line U-251 MG shows positivity in mitochondria. Antibody staining is shown in green.
GRIM19 Antibody - BSA Free Immunohistochemistry: GRIM19 Antibody - BSA Free [NBP1-90050]

Immunohistochemistry: GRIM19 Antibody - BSA Free [NBP1-90050]

Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
GRIM19 Antibody - BSA Free Immunohistochemistry: GRIM19 Antibody - BSA Free [NBP1-90050]

Immunohistochemistry: GRIM19 Antibody - BSA Free [NBP1-90050]

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
GRIM19 Antibody - BSA Free Immunohistochemistry: GRIM19 Antibody - BSA Free [NBP1-90050]

Immunohistochemistry: GRIM19 Antibody - BSA Free [NBP1-90050]

Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
GRIM19 Antibody - BSA Free Western Blot: GRIM19 Antibody - BSA Free [NBP1-90050]

Western Blot: GRIM19 Antibody - BSA Free [NBP1-90050]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
GRIM19 Antibody - BSA Free Western Blot: GRIM19 Antibody - BSA Free [NBP1-90050]

Western Blot: GRIM19 Antibody - BSA Free [NBP1-90050]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4

Applications for GRIM19 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRIM19

The GRIM19 gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transf

Alternate Names

B16.6, CDA016, Cell death regulatory protein GRIM-19, cell death-regulatory protein GRIM19, CI-B16.6, complex I B16.6 subunit, Complex I-B16.6, FLJ58045, FLJ59191, Gene associated with retinoic and IFN-induced mortality 19 protein, Gene associated with retinoic and interferon-induced mortality 19 protein, GRIM-19CGI-39, GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Gene Symbol

NDUFA13

Additional GRIM19 Products

Product Documents for GRIM19 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GRIM19 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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