![Immunohistochemistry-Paraffin: HBsAg Antibody (5C3) [NBP3-07121]](https://resources.rndsystems.com/images/products/HBsAg-Antibody-5C3-Immunohistochemistry-Paraffin-NBP3-07121-img0001.jpg "Immunohistochemistry-Paraffin: HBsAg Antibody (5C3) [NBP3-07121]")
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Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5C3
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Product Specifications
Immunogen
HBsAg (ad/ay) protein
Localization
Cytoplasm
Specificity
This antibody reacts with the 'a' determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for HBsAg Antibody (5C3)
Immunohistochemistry-Paraffin: HBsAg Antibody (5C3) [NBP3-07121]
Immunohistochemistry-Paraffin: HBsAg Antibody (5C3) [NBP3-07121] - Formalin fixed paraffin embedded human infected liver stained with HBsAg antibody(NBP3-07121)Applications for HBsAg Antibody (5C3)
Application
Recommended Usage
Immunohistochemistry
1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HBsAg
Alternate Names
HBsAg, HBV major surface antigen, HBV surface antigen, Hepatitis B Virus major surface antigen, Hepatitis B Virus Surface Antigen, Major surface antigen, protein S
Gene Symbol
S
Additional HBsAg Products
Product Documents for HBsAg Antibody (5C3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HBsAg Antibody (5C3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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