HCN1 Antibody
Novus Biologicals | Catalog # NBP1-20250
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
A synthetic peptide from cytoplasmic region of human HCN1 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including mouse and rat.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for HCN1 Antibody
Western Blot: HCN1 Antibody [NBP1-20250] -
HCN2 subunit knockdown in rat hippocampal CA1 region. A HCN1 and HCN2 expression in CA1 after TGCI (experiments were performed at least four times with similar results). B Distribution of lentivirus in the dorsal hippocampal CA1 region (green; × 40, × 100, scale bars, 100 μm). C Relative expression of HCN1 and HCN2 channels at the protein level in Con-shRNA and HCN2-shRNA rats (experiments were performed at least four times with similar results). **P < 0.01 versus Con group; ##P < 0.01 versus Con-shRNA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32519067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: HCN1 Antibody [NBP1-20250] -
HCN2 subunit knockdown in HT22 neurons. A HCN1 and HCN2 expression in HT22 neurons after OGD/R injury (experiments were performed at least four times with similar results). B Representative photomicrographs of fluorescence produced by EGFP and immunohistochemical staining with anti-HCN2 antibody in HT22 neurons (× 200, scale bar, 200 μm). C Quantitative analysis of HCN2 immunoreactivity (experiments were performed at least three times with similar results). D Relative expression of HCN1 and HCN2 channels at the protein level in Con-shRNA and HCN2-shRNA cells (experiments were performed at least four times with similar results) **P < 0.01 versus control group; ##P < 0.01 versus Con-shRNA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32519067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: HCN1 Antibody [NBP1-20250] -
HCN2 subunit knockdown in rat hippocampal CA1 region. A HCN1 and HCN2 expression in CA1 after TGCI (experiments were performed at least four times with similar results). B Distribution of lentivirus in the dorsal hippocampal CA1 region (green; × 40, × 100, scale bars, 100 μm). C Relative expression of HCN1 and HCN2 channels at the protein level in Con-shRNA and HCN2-shRNA rats (experiments were performed at least four times with similar results). **P < 0.01 versus Con group; ##P < 0.01 versus Con-shRNA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32519067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for HCN1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:300-1:2000
Western Blot
1:300-1:2000
Application Notes
Immunohistochemistry and Western Blot. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31923455).
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: HCN1
Long Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Alternate Names
BCNG-1, BCNG1, EIEE24, GEFSP10, HAC-2
Gene Symbol
HCN1
UniProt
Additional HCN1 Products
Product Documents for HCN1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HCN1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HCN1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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