HIPPI Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16835

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HIPPI Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIPPI. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (83%).

Localization

Cell projection; cilium basal body (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIPPI Antibody - BSA Free

Western Blot: HIPPI Antibody [NBP2-16835]

Western Blot: HIPPI Antibody [NBP2-16835]

Western Blot: HIPPI Antibody [NBP2-16835] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HIPPI Antibody [NBP2-16835]

Immunocytochemistry/ Immunofluorescence: HIPPI Antibody [NBP2-16835]

Immunocytochemistry/Immunofluorescence: HIPPI Antibody [NBP2-16835] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HIPPI Antibody [NBP2-16835]

Immunohistochemistry-Paraffin: HIPPI Antibody [NBP2-16835]

Immunohistochemistry-Paraffin: HIPPI Antibody [NBP2-16835] - Immunohistochemical analysis of paraffin-embedded H661 xenograft, using antibody at 1:500 dilution.

Applications for HIPPI Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIPPI

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

Alternate Names

Dermal papilla-derived protein 8, DERP8, ESRRBL1huntingtin interacting protein-1 interacting protein, estrogen-related receptor beta like 1, Estrogen-related receptor beta-like protein 1, FLJ10147, HIPPIHIP1-interacting protein, intraflagellar transport 57 homolog (Chlamydomonas), intraflagellar transport protein 57 homolog, MHS4R2HIP1 protein interactor

Gene Symbol

IFT57

UniProt

Q9NWB7 (Human)

Additional HIPPI Products

Product Documents for HIPPI Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HIPPI Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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