HIST1H3A Antibody (CL4967) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44249
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL4967
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P68431, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for HIST1H3A Antibody (CL4967) - Azide and BSA Free
![HIST1H3A Antibody (CL4967) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-282202512154157.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]
Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.![HIST1H3A Antibody (CL4967) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-282202512321013.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]
Staining of human kidney shows strong nuclear positivity in renal tubules cells.![HIST1H3A Antibody (CL4967) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-282202512321015.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]
Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.![HIST1H3A Antibody (CL4967) - Azide and BSA Free Western Blot: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-282202513254737.jpg "Western Blot: HIST1H3A Antibody [NBP3-44249]")
Western Blot: HIST1H3A Antibody [NBP3-44249]
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![HIST1H3A Antibody (CL4967) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-282202513332319.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody [NBP3-44249]")
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody [NBP3-44249]
Staining of U-2 OS cells using the Anti-HIST1H3A monoclonal antibody).![HIST1H3A Antibody (CL4967) - Azide and BSA Free Western Blot: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-28220251324553.jpg "Western Blot: HIST1H3A Antibody [NBP3-44249]")
Western Blot: HIST1H3A Antibody [NBP3-44249]
Analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.![HIST1H3A Antibody (CL4967) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-28220251339142.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44249]
Staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.![HIST1H3A Antibody (CL4967) - Azide and BSA Free Western Blot: HIST1H3A Antibody [NBP3-44249]](https://resources.rndsystems.com/images/products/nbp3-44249_mouse-hist1h3a-mab-cl4967-azide-and-bsa-free-282202513381542.jpg "Western Blot: HIST1H3A Antibody [NBP3-44249]")
Western Blot: HIST1H3A Antibody [NBP3-44249]
Analysis in human cell line HeLa.Applications for HIST1H3A Antibody (CL4967) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:10000 - 1:20000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Documents for HIST1H3A Antibody (CL4967) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIST1H3A Antibody (CL4967) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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