Histone H3.3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24697

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Chicken, Drosophila, Equine, Opossum, Zebrafish

Cited:

Avian - Chicken

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H3.3 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 100-136 of human Histone H3.3 was used as the immunogen for the antibody.

Reactivity Notes

Ortangutan (100%), Salmon (100%).

Marker

Marker of Transcriptionally Active Chromatin

Specificity

100 % homologous in human (Histone H3.3A, B, and C).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.3 Antibody - BSA Free

Western Blot: Histone H3.3 Antibody [NBP2-24697]

Western Blot: Histone H3.3 Antibody [NBP2-24697]

Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]

Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]

Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697] - Human small intestine stained with Histone H3.3 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Applications for Histone H3.3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.25-0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H3.3

Alternate Names

H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783

Gene Symbol

H3-3A

Additional Histone H3.3 Products

Product Documents for Histone H3.3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H3.3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histone H3.3 Antibody - BSA Free

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Protocols

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FAQs

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