Viral HIV-1 Gag p24 Antibody (749122)

R&D Systems | Catalog # MAB73602

R&D Systems

Key Product Details

Species Reactivity

Viral

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 749122
View all HIV-1 Gag p24 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # Q77YG1_9HIV1

Specificity

Detects viral HIV-1 Gag p24 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral HIV-1 Gag p24 Antibody (749122)

Detection of HIV-1 Gag p24 antibody by Western Blot.

Detection of HIV‑1 Gag p24 by Western Blot.

Western blot shows lysates of recombinant HIV-1 Gag p24. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Viral HIV-1 Gag p24 Antibody in ELISA Standard Curve.

Viral HIV‑1 Gag p24 ELISA Standard Curve.

Recombinant Viral HIV-1 Gag p24 protein was serially diluted 2-fold and captured by Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Viral HIV-1 Gag p24 Antibody (749122)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73601).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the HIV-1 Gag p24 DuoSet ELISA Kit (Catalog # DY7360-05) for convenient development of a sandwich ELISA or the HIV-1 Gag p24 Quantikine ELISA Kit (Catalog # DHP240) for a complete optimized ELISA.

Western Blot

0.1 µg/mL
Sample: Recombinant HIV-1 Gag p24

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

Q77YG1_9HIV1

Additional HIV-1 Gag p24 Products

Product Documents for Viral HIV-1 Gag p24 Antibody (749122)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral HIV-1 Gag p24 Antibody (749122)

For research use only

Related Research Areas

Infectious Diseases

Citations for Viral HIV-1 Gag p24 Antibody (749122)

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