HMG-CoA Reductase/HMGCR Antibody (JF0981)

Novus Biologicals | Catalog # NBP2-66888

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0981 expressed in HEK293
View all HMG-CoA Reductase/HMGCR Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human HMG-CoA Reductase/HMGCR aa 438-489 / 888. (SwissProt: P04035 Human; SwissProt: Q01237 Mouse; SwissProt: P51639 Rat)

Localization

Endoplasmic reticulum membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMG-CoA Reductase/HMGCR Antibody (JF0981)

Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888] - Analysis of HMGCR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: THP-1 cell lysate
Simple Western: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888]

Simple Western: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888]

Simple Western: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888] - SVG-A whole cell lysate. Antibody at 1:25 dilution. Image submitted by a verified customer review.
HMG-CoA Reductase/HMGCR Antibody (JF0981) Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888]

Western blot analysis on different lysates with NBP2-66888 at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66888 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for HMG-CoA Reductase/HMGCR Antibody (JF0981)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100

Simple Western

1:25

Western Blot

1:500-1:2000
Application Notes
HMG-CoA Reductase/HMGCR antibody validated for Simple Western from a verified customer review. IP: Use at an assay dependent concentration.
See Simple Western Antibody Database for Simple Western validation: Tested in SVG-A whole cell lysate, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 1 review rated 5 using NBP2-66888 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMG-CoA Reductase/HMGCR

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

3-Hydroxy-3-Methylglutaryl-CoA Reductase

Alternate Names

HMGCR, Hydroxymethylglutaryl-CoA Reductase, LDLCQ3

Gene Symbol

HMGCR

Additional HMG-CoA Reductase/HMGCR Products

Product Documents for HMG-CoA Reductase/HMGCR Antibody (JF0981)

Certificate of Analysis

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Product Specific Notices for HMG-CoA Reductase/HMGCR Antibody (JF0981)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for HMG-CoA Reductase/HMGCR Antibody (JF0981)

Customer Reviews for HMG-CoA Reductase/HMGCR Antibody (JF0981) (1)

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  • HMG-CoA Reductase/HMGCR Antibody (JF0981)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: SVG-A whole cell lysate
    Species: Human
    Verified Customer | Posted 07/03/2018
    SVG-A whole cell lysate run at the indicated concentration. Antibody was tested at 1:25 dil.
    HMG-CoA Reductase/HMGCR Antibody (JF0981) NBP2-66888

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