HNF-4 alpha/NR2A1 Antibody (4E2)

Novus Biologicals | Catalog # H00003172-M04

Novus Biologicals
Discontinued Product
H00003172-M04 has been discontinued. View all HNF-4 alpha/NR2A1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4E2
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

HNF4A (NP_000448, 324 a.a. ~ 423 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRP

Specificity

HNF4A (4E2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody (4E2)

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2. Lane 1: HNF4A transfected lysatE (51.6 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of monoclonal antibody to HNF4A on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi or non-transfected control. Blot probed with H00003172-M04. GAPDH (36.1 kDa) used as loading control.
Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in HepG2 (Cat # L019V1).
Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in Jurkat (Cat # L017V1).
ELISA: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

ELISA: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04]

ELISA: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.

Applications for HNF-4 alpha/NR2A1 Antibody (4E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

OMIM

125850 (Human)

UniProt

NP_000448 (Human)

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody (4E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HNF-4 alpha/NR2A1 Antibody (4E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HNF-4 alpha/NR2A1 Antibody (4E2)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail