HPV16 E1+E4 Antibody (HPV16 E1/E4) [mFluor Violet 500 SE]

Novus Biologicals | Catalog # NBP3-08319MFV500

Novus Biologicals

Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry-Paraffin

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HPV16 E1/E4
View all HPV16 E1+E4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human HPV16 E1+E4 fragment (aa 36-41).

Reactivity Notes

Type 16 of Human Papilloma Virus (HPV-16).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for HPV16 E1+E4 Antibody (HPV16 E1/E4) [mFluor Violet 500 SE]

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HPV16 E1+E4

HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Alternate Names

ATP dependent helicase E1, E1, E4, HPV 16 ATP dependent helicase E1, HPV 16 E1, HPV 16 E4, HPV 16 replication protein E1, HPV16 ATP dependent helicase E1, HPV16 E1, HPV16 E4, HPV16 replication protein E1, Human papilloma virus type 16, Human papilloma virus type 16 ATP dependent helicase E1, Human papilloma virus type 16 E1, Human papilloma virus type 16 E4, Human papilloma virus type 16 replication protein E1, Human papillomavirus type 16, Human papillomavirus type 16 ATP dependent helicase E1, Human papillomavirus type 16 E1, Human papillomavirus type 16 E4, Human papillomavirus type 16 replication protein E1, Replication protein, Replication protein E1

Additional HPV16 E1+E4 Products

Product Documents for HPV16 E1+E4 Antibody (HPV16 E1/E4) [mFluor Violet 500 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HPV16 E1+E4 Antibody (HPV16 E1/E4) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HPV16 E1+E4 Antibody (HPV16 E1/E4) [mFluor Violet 500 SE]

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  • Q: I was wondering if the sequence recognized by the antibody TVG 402 (NB100-2766) corresponds to the E1 sequence or to the E4 sequence? As in, would this antibody recognize either E4 or E1 if they are not found as a E1^E4 fusion protein?

    A: According to our product page the immunogen says : Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.HEre is the original paper describing the characterization of this clone (TVG 402): https://www.sciencedirect.com/science/article/pii/004268229290327L?via%3Dihub

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