HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70962

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI1H3

Format

Azide and BSA Free
View all HSPA8/HSC71 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA8(NP_006588) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

70.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3)Azide and BSA Free [NBP2-70962]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3)Azide and BSA Free [NBP2-70962]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.
Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962]

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962]

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8.
Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962]

Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962]

Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3)Azide and BSA Free [NBP2-70962]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3)Azide and BSA Free [NBP2-70962]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Applications for HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSPA8/HSC71

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3).

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

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Protocols

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