HTRA2/Omi Antibody (196C429)
Novus Biologicals | Catalog # NB100-56558
![Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]](https://resources.rndsystems.com/images/products/HTRA2-Omi-Antibody-196C429-Western-Blot-NB100-56558-img0005.jpg "Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 196C429
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Product Specifications
Immunogen
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 335-350 of human HtrA2.
Specificity
This antibody does not cross-react with mouse HtrA2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for HTRA2/Omi Antibody (196C429)
Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]
Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558] - Detection of HtrA2 using an HtrA2 monoclonal antibody. HL-60 cell lysate was probed with HtrA2 antibody at 3 ug/ml and two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2.![Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]](https://resources.rndsystems.com/images/products/HTRA2-Omi-Antibody-196C429-Immunohistochemistry-NB100-56558-img0004.jpg "Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]")
Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]
Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558] - Analysis of HtrA2 using an HtrA2 monoclonal antibody. Probing with HtrA2 antibody shows staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).Applications for HTRA2/Omi Antibody (196C429)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
reported in scientific literature (Lee et al (2003))
Western Blot
2-3 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HTRA2/Omi
Long Name
High Temperature Requirement Protein-2
Alternate Names
Omi, PRSS25
Gene Symbol
HTRA2
Additional HTRA2/Omi Products
Product Documents for HTRA2/Omi Antibody (196C429)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HTRA2/Omi Antibody (196C429)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for HTRA2/Omi Antibody (196C429)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
