Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is secreted from adipocytes and has been implicated in energy homeostasis and obesity.
Human Adiponectin/Acrp30 Antibody
R&D Systems | Catalog # MAB1065
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Direct ELISA
Cited:
ELISA Detection, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 166128
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Adiponectin
Glu19-Asn244
Accession # Q15848
Glu19-Asn244
Accession # Q15848
Specificity
Detects the globular domain of human Adiponectin/Acrp30 in direct ELISAs. In direct ELISAs, no show cross-reactivity with recombinant mouse Acrp30 or recombinant rat Acrp30 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Adiponectin/Acrp30 Antibody
Application
Recommended Usage
Direct ELISA
This antibody can be used at 0.5‑1.0 μg/mL with the appropriate secondary reagents to detect human Adiponectin/Acrp30. The detection limit for recombinant human Adiponectin is approximately 3 ng/well.
Reviewed Applications
Read 1 review rated 4 using MAB1065 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Adiponectin/Acrp30
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
UniProt
Additional Adiponectin/Acrp30 Products
Product Documents for Human Adiponectin/Acrp30 Antibody
Product Specific Notices for Human Adiponectin/Acrp30 Antibody
For research use only
Related Research Areas
Citations for Human Adiponectin/Acrp30 Antibody
Customer Reviews for Human Adiponectin/Acrp30 Antibody (1)
4 out of 5
1 Customer Rating
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 12/06/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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