Human AIF-1/Iba1 Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: AIF-1/Iba1

Allograft-inflammatory factor 1 (AIF-1), also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed in activated macrophages and is important for the proinflammatory response to ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular smooth muscle and endothelial cells in these areas where it promotes vascular remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue during transplant rejection. It also promotes the proliferation of breast cancer cells and in vivo tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the N-terminal 54 aa of isoform 3. Isoform 2 lacks
aa 1-54 as well as aa 121-147 but also has a 66 aa insertion at position 65.

Product Datasheets

Related Research Areas

• Calcium-binding Proteins and Related Molecules
• Microfilament-associated Proteins
• Microglia Steady-State Markers
• Mouse Tissue-Resident Macrophage Markers