Human Apolipoprotein B/ApoB Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3556X Datasheet / COA / SDS

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AF3556X-100UG

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Human Apolipoprotein B/ApoB Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein B/ApoB

ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100