Human Apolipoprotein B/ApoB Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB41241N Datasheet / COA / SDS

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FAB41241N-100UG

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Human Apolipoprotein B/ApoB Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Apolipoprotein B/ApoB in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 989529

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein B/ApoB

Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 amino acids (aa) in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48, and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (512 kDa). Amino acids 1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297 aa), B37 (1728 aa), B39 (1799 aa), and B48 (2152 aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and dog ApoB-100, respectively.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100