Human APRIL/TNFSF13 Antibody (670840)

Name: Human APRIL/TNFSF13 Antibody (670840)
Brand: R&D Systems
SKU: MAB8843
Price: 479 USD
Availability: InStock
Rating: 3 (1 reviews)

R&D Systems | Catalog # MAB8843

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Citations (1)

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 670840

View all APRIL/TNFSF13 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293EBNA-derived recombinant human APRIL/TNFSF13
Ala105-Leu250
Accession # O75888

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse APRIL, recombinant human (rh) BAFF, rhEDA-A2, rhEDA, rhFas Ligand, rhLIGHT, rhLT a1/b2, rhLT a2/b1, rhOX40 Ligand, rhTNF alpha, rhTRAIL, rhTRANCE, rh4-1BB Ligand, rhVEGI, or rhGITR Ligand is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human APRIL/TNFSF13 Antibody (670840)

Detection of April in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes stimulated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for three days followed by a 3 hour incubation with monensin were stained with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8843) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB004). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human APRIL/TNFSF13 Antibody (670840)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood monocytes, treated with Recombinant Human IL-4 (Catalog # 204-IL), Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) and monensin, fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 1 review rated 3 using MAB8843 in the following applications:

ELISA (1 Review)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: APRIL/TNFSF13

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat); 102126587 (Cynomolgus Monkey)

Gene Symbol

TNFSF13

UniProt

O75888 (Human)

Additional APRIL/TNFSF13 Products

Product Documents for Human APRIL/TNFSF13 Antibody (670840)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human APRIL/TNFSF13 Antibody (670840)

For research use only

Related Research Areas

Cancer Biomarkers

Regulation of Apoptosis by TNF Superfamily Members

TNF Superfamily

Citations for Human APRIL/TNFSF13 Antibody (670840)

Customer Reviews for Human APRIL/TNFSF13 Antibody (670840) (1)

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Name: Anonymous

Application: ELISA

Sample Tested: Recombinant protein

Species: Human

Verified Customer | Posted 09/09/2024

dot blot image

My plan was to use MAB8843 detect 5860-AP in my ELISA. Although the ELISA did not work as expected, I was able to used dot blot to check for compatibility between MAB8843 and anti-mouse secondary antibody as suggested by the Technical Team. Attached is the dot blot image. Note: the secondary antibody is from a different manufacturer.

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