Human BACE-2 Ectodomain Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB4097V Datasheet / COA / SDS

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FAB4097V-100UG

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Human BACE-2 Ectodomain Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BACE‑2 Ectodomain in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse BACE-2 is observed and no cross-reactivity with recombinant human (rh) BACE, rhCathepsin D, or rhCathepsin E

Source

Monoclonal Rat IgG2a Clone # 391017

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human BACE-2
Ala21-Tyr473
Accession # Q9Y5Z0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BACE-2

BACE-2 ( beta -site APP cleaving enzyme 2; also Asp-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both in- and outside the CNS, and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear; if it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa 329‑378, while a second shows an 18 aa substitution for aa 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE-2.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Entrez Gene IDs

25825 (Human); 56175 (Mouse); 288227 (Rat)

Alternate Names

AEPLC; Asp 1; ASP1; ASP21; Aspartic-like protease 56 kDa; Aspartyl protease 1; BACE2; BACE-2; beta secretase 2; beta-secretase 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2; Beta-site amyloid precursor protein cleaving enzyme 2; Beta-site APP cleaving enzyme 2; beta-site APP-cleaving enzyme 2; beta-site APP-cleaving enzyme 2, EC 3.4.2310ALP5656 kDa aspartic-like protease; CEAP1; Down region aspartic protease; Down syndrome region aspartic protease; DRAPBAE2; EC 3.4.23; EC 3.4.23.45; memapsin-1; Membrane-associated aspartic protease 1; theta-secretase; transmembrane aspartic proteinase Asp1